Fig. 3. EC-specific HDAC2 overexpression protects impaired endothelial-dependent vascular relaxation in atherogenic mice. Aortic rings were harvested from PCSK9 AAV-injected C57BL/6 (WT) and HDAC2-Tg mice fed with a high fat diet (HFD). Dose-response effects of acetylcholine (A) and sodium nitroprusside (SNP) (B) on vascular relaxation were determined by wire myography. (C) Acetylcholine dose response was also measured in the presence of 100 µM L-NAME. *p<0.05 vs WT HFD, n=6.